Affinity precipitation of enzymes
نویسندگان
چکیده
منابع مشابه
Affinity chromatography of nuclear enzymes.
There are a number of nuclear enzymes that bind nicotinamide, thymidine or nucleotides. We use this fact to prepare affinity columns for the purification of nuclear enzymes. In particular the nuclei of eukaryotic cells have an enzyme that catalyses the synthesis of the homopolymer poly(ADP-ribose) from NAD. Isolation and partial purification has been achieved (Yamada et al., 1971 ; Ueda et al.,...
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Background: Increase of protein purity is a serious challenge in the production of recombinant therapeutic proteins. For this purpose, several strategies have been employed to purify the target protein, among which the affinity chromatography-based purification methods and tagged proteins such as Ni-NTA are common and but costly. Therefore column-free purification techniques, such as using elas...
متن کاملA thermal responsive affinity ligand for precipitation of sialylated proteins
We report here the development of a thermal responsive affinity ligand specific to sialic acid, sialic acid containing oligosaccharides, glycoproteins, and other sialylated glycoconjugates. The ligand is a fusion protein of 40 repeats of pentapeptide of an elastin like polymer (ELP) and the 21 kD sialic acid binding domain of a Vibrio cholera neuraminidase (VCNA). For cost-effective synthesis, ...
متن کاملAffinity precipitation of Aspergillus niger pectinase by microwave-treated alginate.
Affinity precipitation is a simple, single plate separation process in which the complex of a smart macroaffinity ligand with the target protein (from a crude broth) can be selectively precipitated by application of a suitable stimulus. Alginate is a copolymer of guluronic acid and mannuronic acid residues and precipitates with Ca(2+) ions. It was found to bind to pectinase present in a commerc...
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The use of ligand-modified phospholipids solubilized in aqueous solution by nonionic surfactant for affinity precipitation of proteins is described. Avidin was precipitated by contact with solutions in which dimyristoylphosphatidylethanolamine (DMPE) functionalized with biotin (DMPE-B) was solubilized in octaethylene glycol mono-n-dodecyl ether (C12E8) solutions. The nonionic surfactant solubil...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1979
ISSN: 0014-5793
DOI: 10.1016/0014-5793(79)80211-2